"Illumina/TruSight Tumor 15 MiSeq Kit/OP-101-1001/1 Ea

" OP-101-1001 Illumina 產(chǎn)品編號： OP-101-1001美  元  價(jià)： $0.00會  員  價(jià)： 待定品       牌： Illumina產(chǎn)       地： 美國公       司： Illumina, Inc.產(chǎn)品分類： 其它>其它試劑>芯片公司分類： Clinical Research Products "Product Highlights:

TruSight Tumor 15 uses next-generation sequencing (NGS) technology to provide a comprehensive assessment of 15 genes that are commonly mutated in solid tumors. It accurately detects low-frequency variants from 20 ng of starting DNA and is optimized for formalin-fixed, paraffin-embedded (FFPE) tumor tissue.

View the Gene List

Featuring a rapid workflow that can be easily integrated into lab procedures, this panel offers a single assay for accurate, economical, and rapid analysis of solid tumors.

This TruSight Tumor panel offers:

Comprehensive workflow: Assess 15 genes with one simple workflow instead of single, iterative gene testing with polymerase chain reaction (PCR)

Efficient: Rapid turnaround with only 3.5 hours of hands-on time, going from DNA to data in approximately 36 hours

Relevant gene content for solid tumors: Somatic variants selected from relevant industry guidelines1,2, key opinion leaders3,4, and pharmaceutical research

Sensitive variant detection from low DNA input: Accurate somatic variant detection of 5% allele frequency using 20 ng DNA from FFPE tissue samples

TruSight Tumor 15 Sample Datasets

3 human reference samples and 5 FFPE-exacted DNA samples from lung, colon, melanoma, and breast tumors were prepared using TruSight Tumor 15. These libraries were sequenced on the MiniSeq System using a high output kit at a 2 x 150 bp read length configuration with dual indexing. The total yield was 7.6 Gb with 94.9% of bases at or above Q30.

Browse the data in BaseSpace Sequence Hub:

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Access to this data requires a BaseSpace Sequence Hub login.

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Specifications:

Assay Time 7 hours

Hands-On Time 3.5 hours

Input Quantity 20 ng

System Compatibility MiSeq,MiSeqDx in Research Mode,MiniSeq

Specialized Sample Types FFPE,Low Input

Technology Sequencing

Variant Class Somatic Variants,Insertions-Deletions (indels)

Species Category Human

Cancer Type Solid Tumor

Method Amplicon Sequencing,Targeted DNA Sequencing"

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